Can you advise me on the high efficiency transfection of the cells in the suspension?

02 February 2015 1 3K Report

I am working with the transformed bovine lymphocytes. These cells are in the suspension and I am planning to transfect these cells with the gene of my interest. Cell in suspension is often hard to transfect. Once I tried to transfect them with the reagent (Lipofectamin LTX and plus) which I had used for Vero 2-2 cells (adherent cells). But that didn't work. So, I was wondering if someone has tried to do anything like this. If yes, then can you write me an answer down below?

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Science method

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