Neeta Shrestha

3 Questions 5 Answers 0 Followers

Questions related from Neeta Shrestha

Can peripheral blood lymphocytes survive in a culture medium for a day or two without any supplements?

I am trying to do some experiments on peripheral blood lymphocytes. And for that purpose I need to keep these primary cells alive in culture medium for a day or two. Does anybody know if they will...

12 December 2015 1,603 7 View

Can you advise me on the high efficiency transfection of the cells in the suspension?

I am working with the transformed bovine lymphocytes. These cells are in the suspension and I am planning to transfect these cells with the gene of my interest. Cell in suspension is often hard to...

02 February 2015 3,144 1 View

What is the time point for the observation of global impact of viral miRNAs on transduced cells?

My experiment is related with miRNAs. I would like to see the effect of miRNA on transduced cells. So the idea is that miRNA after causing obstruction of protein production should show subsequent...

09 September 2014 2,953 4 View