Can we use tissue samples in RNA later stored immediately in -80C for proteomics assays?

More Pradeep B Patil's questions See All

Does skin scraping in dead animal will have any significance?

I have a found dead animal in sentinel mice colony. Does skin scrapping is a valuable in such animals or not? Will the ecto-parasites present after six hours of death?

09 October 2017 9,346 3 View

Is there any reference for neurovirulence test of chikunguniya virus in monkeys?

I need a reference for MNVT in nonhuman primate brain/ lumbar region for chikunguniya , which is difficult to find , so pls guide me.

08 September 2017 8,645 1 View

Is there any reference for testing of immunogenicity of mumps virus in nonhuman primate?

I am looking for a reference on mumps virus immunogenicity testing in nonhuman primate, pls guide me on it.

08 September 2017 7,896 3 View

Does long acting penicillin single dose will have any effect on animal's immune response for implanted scaffold?

I would like to test immune response of an animal model for a biological scaffold. Can I use single dose of antibiotic during implantation procedure? Will it affect the study?

01 February 2016 4,936 0 View

What is the difference between cell engraftment and repopulation?

06 July 2014 9,236 4 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Ajit kumar Roy

pl see the interesting article in link

Article Comparing the proteome of snap frozen, RNAlater preserved, a...

Hong Wang

If I understand you right, you want to apply the limited precious sample for both proteomic and RNA analysis. I remember there is one publication on extract both protein and RNA from the same sample. Just google the paper out, it may help.