I want to screen the antibacterial activity of the plant extract. and i will be used tetracycline or ampicilin drug as the positive control. However, the one that i have right now is already expired. Can I still use it in the test? Thank you.
Yes you can if you aware of quality control policy of antibiotic and other biological materials used in medical laboratories.You obviously should know the standard zone of inhibition for antibiotic disc before expire.You must use the expired antibiotic disc with reference bacterial strain and measure the zone then decide if it is still viable as new or not before utilizing this disc in clinical assessement.
Dear
my answer is no. it is because can anyone eat expired medicine and it can show other effects on bacteria also. so my suggestion is you take new drug for your study.
Yes you can if you aware of quality control policy of antibiotic and other biological materials used in medical laboratories.You obviously should know the standard zone of inhibition for antibiotic disc before expire.You must use the expired antibiotic disc with reference bacterial strain and measure the zone then decide if it is still viable as new or not before utilizing this disc in clinical assessement.
Most antibiotics -if properly stored- have a longer shelf-life than the one given on the package. So I agree with Mohemid: check the zone of inhibition with a QC-strain or a strain you've used before to make sure the discs are OK. Besides, if you're using the discs as positive control I guess a small variability won't be a big deal. It'd be a wholly different thing if it had been a sample in a clinical lab...
Yes you can. I agree with Mohemid but be aware that you should use the reference bacterial strains which are preserved in lyophilized form or the frozen one that have not experienced many thaw cycles in order to avoid any changes in antibacterial sensitivity. It is not acceptable the ones that are re-cultured more than 5 times.
Yes I agree with Mohamed as the discs have a longer shelf -line than given in package But you can do verification by assays both of expired discs and non expired and see if there's differences.
if you can compare the results with non expired drug you can use since some drugs can work for a short period after expiration date if they were well stored
If your lab is working under ISO quality control, you're not even allowed to keep them in you refrigerator. As to comparing of expired disks to non-expired ones this may be acceptable but ask yourself the following question: if you were (God-forbid) the patient, would you like to be treated with an antibiotic whose activity was determined by an expired disk even a validated one?
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