Fairuz Iman

7 Questions 4 Answers 0 Followers

Questions related from Fairuz Iman

Why did my sample, with the same OD as the standard, not develop the same colour as the standard in NO assay using Griess reagent?

I'm testing for NO assay, using Griess reagent. As stated in the protocol, I did serial dilution of the standard. Upon adding both reagents, the OD obtained in standards (with known concentration)...

01 December 2015 9,788 6 View

Why my resolving buffer form a crystalized like substances when stored at 4 degree?

Every time I store my resolving buffer at 4 degree, the next day a crystallized like substances will appear at the bottom of the bottle. Why is it happening? is it because of pH? I already...

19 September 2015 8,018 4 View

Can i used commercial disc as positive control in agar well diffusion method?

I want to screen antimicrobial activity of certain extracts, and i choose to do agar well diffusion method. For positive control, can I used the standard antibiotic and placed it on the agar,...

03 July 2015 8,650 5 View

Can we use the expired antibiotic disc for disc diffusion test?

I want to screen the antibacterial activity of the plant extract. and i will be used tetracycline or ampicilin drug as the positive control. However, the one that i have right now is already...

30 March 2015 6,461 9 View

After drying the sample, how long can it be stored before it will be extracted ?

I stored them at -20 degree Celsius. What happens if I store them for a long time before the extraction starts?

11 March 2015 4,469 4 View

What if I skip the filtration step and concentrate the sample during aqueous extraction of fruit pulp?

during boiling process, the extract already become too saturated and difficult to be filtrated. hence, i skip the filtrating and concentrating steps and proceed with the freeze drying step. is...

08 March 2015 5,362 5 View

What is the time requirement for drying plant extract in a 50 degree incubator that will affect the extraction result?

For example, one day and third day incubation. How can I determine that the extract can be proceed with subsequent procedures? thanks in advanced!

05 March 2015 1,861 8 View