Can we use sodium nitroprusside dihydrate instead of sodium nitroprusside for NO scavenging assay?

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What can be the alternate buffer for TAPS (PH 8.5) in Post tagmentation Wash step in Cut & Tag experiment ?

I am performing Cut & Tag experiment. I was wondering if there is any alternative/replacement for TAPS pH 8.5 in single tube Tagmentation and pA-Tn5 release ( DNA purification) step of Cut and...

02 April 2024 4,214 0 View

How much of primary antibody do you add on slides in immunohistochemistry(IHC) / Immunocytochemistry (ICC)?

Most of the Antibody company (having pr. Ab conc. range 0.2 mg/ml - 0.5mg/ml) websites suggests dilution around 1:200, but it seems not staining or faintly staining. What is the hand on experience...

28 March 2024 259 3 View

Can i know how to find parameters for power system generation and transmission with integration of BESS?

I am doing a final year project for my degree and im struggling to find for the parameters for the power system that resembles the power system network of Malaysia (Tenaga Nasional Berhad). This...

12 March 2024 4,979 1 View

Why do I get minor electric shock in my laboratory when touch something metallic?

Sometimes when I touch my colleague or metal bodies. i fell shock with some minor electric sound.

27 February 2024 1,191 3 View

How to separate dead cells from live cells when propagating suspension cells?

I am growing small cell lung cancer suspension cells. How do I separate dead cells from live cells while splitting the cells after confluency in flask?

28 January 2024 8,818 6 View

How to do multiple data sequential Rietveld refinement in TOPAS?

I can do Rietveld refinement of single multiple phase data, but I am not able to do multiple data sequential Rietveld refinement. I am using Topas V7.

21 January 2024 2,083 0 View

Why does the transmittance % in FTIR become more than 100% (Opposite direction)?

I did FTIR of a reactive dye's powder. But I see that the transmittance of 3 peaks went opposite direction of normal peaks and eventually the transmittance percentage became more than 100%. What...

17 December 2023 7,093 2 View

Does a thinner intrinsic layer in a 'p-i-n' solar cell, compared to the diffusion length, lead to increased or decreased charge carrier recombination?

As we know, when the thickness of any layer in a p-n junction solar cell exceeds the carrier diffusion length, the photo-generated carriers recombine before reaching the charge transport layer....

15 December 2023 4,930 3 View

How to calculate polymorphic information content (PIC) value for SCoT marker data? Which software helps to calculate it?

I am trying to evaluate the genetic homogeneity of wild grown mother plant and tissue culture raised plants of Piper sp. using SCoT and other dominant markers.

06 December 2023 1,837 0 View

Does enthalpy of chemical sorption reaction is a strong function of pressure and/or temperature for soprtion of ammonia in halide salt?

In most of the literature the enthaply of formation/reaction is chosen to be an constant. Some researchers claimed that in a given pressure range (say: 10 bar) this parameter can be chosen to be a...

07 November 2023 8,839 0 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How can I calculate IC50 in DPPH test by using graph pad prism software?

How can I calculate IC50 in the DPPH test using graph pad prism software? or suggest a manual method and other software to calculate the IC50 value.

06 August 2024 8,848 3 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,830 1 View

How to assess the viability and manage debris in fish primary cell cultures?

Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...

10 July 2024 4,918 2 View