Could you suggest me the optimized procedure for tagging of Sepharose with Protein A?
Need to know the impact of mobile financial services on financial inclusion.
04 May 2024 939 5 View
How can I ensure random sampling for customer surveys when the sampling frame is unavailable but needs to run a regression?
31 August 2023 406 2 View
I am trying to write a critical review based work. For defining some concepts I need to cite a book chapter. And for analysing findings I need to cite three conference papers. Can I use book...
05 July 2023 3,787 2 View
Hi, Is there any simple way which can draw organic reactions mechanism in the articles with out drawing steps one bye one ?
20 April 2023 3,672 2 View
Greetings! I hope this message finds you well. I would be very grateful if you please provide your valuable feedback regarding the scope, feasibility, and methodology of the following research...
15 February 2023 317 1 View
Hi I have question about using method and basis set for optimization: Is it correct? for example I use hf/3-21g for optimization my molecule and after that I use optimize molecule and then put (...
11 February 2023 2,355 2 View
If I want to set my calculation in diffrent temprature and calculate for example gap of homo lumo for organic campound whats is the formula ? it is correct ? b3lyp/6-311++g** pop=full...
08 February 2023 5,974 0 View
What is diffrent between mulliken charge and natural atomic charge in gaussian calculation result ? when we talk about mulliken charge of the for example para bromo benzaldehyde after we...
07 February 2023 8,457 0 View
What is the best method to calculate the HOMO LUMO GAP in solvent condition and with out solvent condition ?
04 February 2023 3,397 6 View
my main question is about which HOMO LUMO energy is correct ? 1-when calculate in the gaussian and after that we can see alpha virt and alpha oct in the notepad ? 2-or when we open the out file...
03 February 2023 793 2 View
Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...
18 July 2024 1,579 2 View
I am working on centrosomal proteins. I overexpressed my POI and then I did SNAP capture pulldown followed by mass spec and I got approximately 700 protein hits. I am trying to validate specific...
03 July 2024 5,701 0 View
I am purifying RNAse E in E. coli (118 kDa). I cloned the rne coding sequence on pET28a with a His tag at the N-terminus. The protein overexpressed fine (see attached figures). The binding buffer...
24 June 2024 6,287 4 View
We are currently interested in performing a stimulation protocol on brain slices in order to perform western blotting or immunoprecipitation. However, we do not know how many 300 um slices would...
12 May 2024 6,527 1 View
Hi All, I am analying the protein-protein interaction in an heterologous system by proximity ligation assay. I over-express one of the proteins, which is a cytosolic protein, and I am trying to...
29 April 2024 2,653 1 View
I am planning an immunoprecipitation experiment using Mouse monoclonal [11C9] to Mannan Binding Lectin/MBL (ab26277) antibody to immunoprecipitate for MBL2 in human serum. There are many protocols...
26 March 2024 3,510 4 View
Can anyone recommend a "good" general phospho-serine and/or phospho-threonine antibody for use after immunoprecipitation in a western blot? There are a lot of expensive offers online with...
22 February 2024 1,080 0 View
Note: Serum isolated extracellular vesicles using immunoprecipitation (magnetic beads)
20 February 2024 4,894 1 View
Hi! I am new to doing Co-IP's and needed help troubleshooting. In short, I have been trying to compare the interaction between Protein A & B and mutated Protein A (mut-A) and B. I know from...
20 December 2023 8,727 0 View
Hello, I want to figure out can I still use my strep-tactin sepharose resin(IBA) if they are dried out for 3 days?
26 November 2023 3,763 1 View