Can we use same mobile phase for detection of 5 HMF and glucose, fructose for hplc?

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During my work in the laboratory?

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Aref Wazwaz

Dear Ganesh Parsai . See the following useful link: https://dergipark.org.tr/tr/download/article-file/612383

Kindly see also the following useful link: Article Determination of 5-hydroxymethylfurfural after Winkler and b...

Also check please the following useful RG link: Article Fast Detection of 5-Hydroxymethylfurfural in Dulce de Leche ...

The following link is also very useful: https://hal.archives-ouvertes.fr/hal-00892181/document

Visit also the following useful link: https://sedere.com/honey-quality-testing-method-using-hplc-uv-sedex-lt-elsd/

Wolfgang Schechinger

That's easy to test. Choose your favorite mobile phase and inject each substance in its pure form, one at a time. You'll need to do that anyway, in order to confirm the identity of your analytes. If a substance does not run satisfactory or does not separate well enough, adapt your solvent.