Hi, we have some problem to extracting DNA of Gram-positive bacteria since we must do it for approx 200 isolates, can we use NaOH method for extracting the genomic DNA or any other rapid method can be used?
Hi, let's use bead extraction method, it is quick and economic, we have used it for many members of Gram positive bacteria, it has been working fine. Please find the protocol at : http://www.ncbi.nlm.nih.gov/pubmed/15935495