I have performed luciferase assay and I want to do western blot using the lysates.
Dear Suruchi,
It is possible to use cell lysate for blotting after luciferase assay.However, your protein lysate might be digested by proteases as a result of manipulation and lysis at room temperature. For this purpose, it might be risky to do so.
Hope it helps.
Best,
Hamadi
Yes, for the reasons geiven by Hamadi, if the lumescience is detected, it may not be of the expected molecular weight.
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