MANY AUTHORS HAVE REPORTED DIFFERENT INCIDENCE AND TUMOR LATENCY FOR RAT MAMMARY TUMORS. CAN WE USE BOTH CARCINOGEN AT DIFFERENT TIME INTERVAL TO INDUCE TUMORS FAST WITH BETTER INCIDENCE?
Given that both N-Nitroso-N-methylurea (NMU) and 7,12-Dimethylbenz(a)anthracene (DMBA) belong to mutagen chemicals and contribute to the initiation, some kinds of promotion to selectively expand the clone with mutation are considered to be required. Why not use TPA?