What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

How to export ZEN 3D time series?

I want to export 3D time series movie from ZEN. I found that I can only export one 3D image with its X or Y rotation instead of 3D-time series? I tried to use Fiji with the function of Z...

07 February 2021 5,365 1 View

(Scale request) Anyone has the Malay version of Eating Attitude Test-26/40 (EAT-26/40)?

Dear Researchers, My research team is conducting a eating disorder related research in Malaysia and we would like to use the Malay translated version of EAT26/40. I have tried to email the...

24 January 2021 8,925 2 View

Difficult to silence gene?

I have been trying to silence a gene of interest but I could not get silencing efficiencies beyond 50% My method of detection is via RT-qPCR and samples were extracted 48hrs post transfection. Are...

09 January 2021 3,404 4 View

HPLC separation for methyl salicylate, camphor, thymol and menthol?

I have been recently trying to develop a method to separate the above compounds. Most methods tried were adapted from literature using RP C8 or C18 column with ACN/MeOH and buffered water at pH 2...

28 December 2020 4,426 6 View

How to extract the different components at a specific frequency between two spectra?

Dear everyone: Hello! Sorry that my question may take up your valuable time. Recently I have a technical problem in the data processing. We have conducted a series of wind tunnel tests in order...

18 December 2020 8,847 2 View

There are prominent crystal formation in my cell culture dish, what are the possible causes and how to get rid of them?

17 December 2020 3,093 4 View

Has anyone extracted plasmid DNA from the Dmp1-GFPtopaz plasmid?

I just bought a plasmid from addgene, the name is DMP1-GFPtopaz. However, I could not extract the vector DNA by using Qiagen DNA purification kits from the bacteria. the bacteria growth was very...

05 November 2020 2,458 3 View

What corporate social responsibilities do social media companies have in relation to the use of psychographic profiling in advertising ?

What are the responsibilities of CSR ? What social media companies have done to protect the privacy or information of their users ?

12 October 2020 433 5 View

Testing significant differences between proportions when subjects overlap?

I am a bit stuck with this. Say for example there is a species of bird with variable colours, and they can present as red, blue, green or a combination (red-blue, red-green, blue-green,...

01 October 2020 3,761 3 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Are there any open-source datasets containing physiological information about exercises to be used for an OR-model?

I have just recently started a new "weekend project" in addition to my master's studies and I am looking for a data-set. I would like to use some Operations Research to design an optimal gym...

22 February 2021 8,023 1 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

Mixed ANOVA with some normal and some non-normal data?

I am looking to run a 2x5 Mixed ANOVA to examine Sensory Processing Sensitivity in Highly Sensitive People (HSP) and Non_HSP. My Between-Subjects factor is HSP and Non-HSP. All participants tested...

11 February 2021 1,445 6 View

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View

Is there any publication about the effect of basketball and volleyball on body height?

Many people believe that some stretching sports with vertical jump such as basketball and volleyball can increase the body height. On the other hand, others do not agree with this argument. The...

02 February 2021 281 6 View

Whic Type of Thermal Camera is Good to use in animal research?

Hello dear researchers I want to use a thermal imaging camera to livestock study. I wonder which type to use. If you know or used a good type please comment or insert its online link in...

29 January 2021 4,163 4 View