Can we use amyl alcohol instead of iso-amyl alcohol (with chloroform) for DNA isolation?
I am not an expert in this field, but I am very interested and have researched to find an answer. I received some assistance from tlooto.com for this response. Could you please review the response below to see if it is correct?
Yes, amyl alcohol can be used in place of iso-amyl alcohol for DNA isolation with chloroform, but it may affect the efficiency of the process. Both amyl and iso-amyl alcohols help reduce foaming and aid in phase separation. Iso-amyl alcohol is preferred due to its higher efficiency in preventing emulsions, leading to clearer phase separation and potentially higher DNA yield and purity [1]. Differences in chemical structure between the two alcohols might impact the overall performance, so preliminary tests are recommended to compare their effectiveness in your specific protocol [2].
Reference
[1] Pappa-Louisi, A., Sotiropoulos, S., & Balkatzopoulou, P. (2008). Mobile Phase pH, Column Temperature, and Eluent Flow Rate Effects on Separation and Fluorescence‐Electrochemical Detection of OPA Derivatives of Amino Acids in Reversed‐Phase Liquid Chromatography. Journal of Liquid Chromatography & Related Technologies, 31, 1434 - 1447.
[2] Intisar, A., Kiazolu, J. B., Wang, Y., Zhang, L., & Zhang, W. (2012). EFFECT OF MOBILE PHASE COMPOSITION AND pH ON HPLC SEPARATION OF RHIZOME OF POLYGONUM BISTORTA. Journal of Liquid Chromatography & Related Technologies, 35, 977 - 987.
Thank you @ Park Sowon for showing interest. Maybe your answer is correct because amyl alcohol is a mixture of different isomers. But my question is, is it practically possible to extract the DNA using amyl alcohol?
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