Dear Rajendra!

Please You look at this publication:

https://academic.oup.com/humrep/article/19/4/948/2913610

The second series of experiments, the shorter protocol, which used DMSO as the cryoprotectant, started like the long protocol but, instead of cooling until –80°C, the slow cooling was halted at –40°C. The cryotubes were then plunged into liquid nitrogen and stored.

For thawing, we compared thawing in a 37°C water bath versus in ice water (rates of 250 or 50°C/min, respectively). The cryoprotectant was diluted as described earlier, after which both concentration and viability of the cell suspension were evaluated.

Article Validation of an automated technique for ovarian cortex diss...

Freezing-thawing and isolation procedure for ovarian tissue