Rajendra Pangeni

4 Questions 10 Answers 0 Followers

Questions related from Rajendra Pangeni

Can we solate viable cells from frozen xenograft tissues?

I am planning to isolate cells from frozen xenograft tissues (stored in liquid nitrogen). The tissues are stored in 10% DMSO, 30%FBS and 60% RPMI with Penn/Strep. These tissues were frozen with a...

15 December 2020 4,243 1 View

Why are not my cells being infected/transducted with lentivirus eventhough the transfection efficiency is really good?

I transfected GipZ lentiviral shRNA with packaging plasmids psPAX2 and VSVG into 293T cells. I also transfected LentiCRISPRv2GFP (lentiviral based GFP containing CRISPR from Adgene). The...

16 January 2018 6,423 2 View

Why isn't there any knockdown even after a good transfection and infection rate?

hi guys, I tried to transfect lentiviral based shRNAs into 293T cells and subsquently infect into stem cells. My transfection and infection looks 70% and 90% positive respectively based on GFP...

24 March 2016 6,121 2 View

Can we look at miRNA expression using general RT PCR without the need of qRT PCR?

I would like to look at the expression of a single miRNA. At this point, I would be inclined to look at miRNA expression by general RT (similar to RT for any other mRNA for a gene) without going...

29 September 2014 1,197 3 View