Presence of protein in a cell line that is used as a negative control for that specific protein?

I have quite an absurd issue. for the information: 1) I have a cell line (K), that i have seen in literature being used as a negative control for my protein (P). However, for me the cell line...

30 May 2019 3,213 3 View

Different genotyping result for the same sample under same conditions?

27 February 2019 331 13 View

Total RNA stability at room temperature (+25 C)?

Hello everyone, I'm facing a problem regarding my pure extracted RNA samples, I extracted them from tissues of rats' livers and kidneys immersed in RNAlater, using the promega SV isolation...

04 November 2017 3,737 5 View

Quality costs in different phases of slip casting?

05 October 2016 5,317 3 View

Comparing different qPCR amplicons, how to account for different length of amplicons when using SyberGreen?

Dear all, I performed several qPCR, which also worked fine, however, now during the evaluation I encountered an issue. My problem is that due to the restriction when choosing primers, I ended up...

05 August 2014 3,406 2 View

For ELISA, which capture/coating AB concentration should I use?

I am developping an ELISA, which seems to work, although I am puzzled by the titration of the capture AB. The Assay: All samples were in dublicates, volume used 50ul, except 150 for blocking,...

08 April 2014 6,566 3 View

Is there any evidence that links bacteria with oral cancer?

Certain microbes have been linked with oral cancer like HPV and syphilis. Recent research suggests that anaerobic bacteria may be linked with colon cancer.

26 August 2013 698 24 View

I focus for a training course

I'm a PhD student and I'm working on "Invasive Alien Plants in Tunisia: invasiveness and management strategies." I hope to have the opportunity to benefit from a training period in a laboratory...

17 December 2012 8,704 2 View

Can we modify the pre designed Deep networks like googlenet and alexnet to use them for signal classification?

Signal classification using Deep Learning

01 January 1970 9,370 4 View

What affects will the internet of things (IoT) have on infrastructure and smart cities region?

Is the competencies of the clever grid, smart homes, and its combined with IoT components in other public utilities, including roadways, sewage, and water transport can make a contribution to...

01 January 1970 7,751 5 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View