I tried to extract DNA from a known population using chloroform:isoamylalcohol. The yield i get differs each time and there is no correlation between cell count and DNA concentration. Is there any literature of method to know this?
I agree with Zx methods you are using for dna extraction can cause the difference.
Also, the method of measuring for DNA is important. I normally use nanodrop, and I run on agarose gel to see if they correlate. Sometimes nanodrop measurement is not correlated with gel picture.
Suggestion: try to run dna on gel to see how they look like, also using replicates can help to find a correlation.
good luck in your research,
Ali
It also depends on the metabolic state the cells are in when you harvest them for DNA extraction. If stationary for a longer time many cells will be quiescent or even dead. Additionally , the methods that are used for isolating DNA from bacteria can have a quite high variance in yield.
Cheers, Chrstian
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