Can we isolate RNA from cells after performing ELISA?

More Deeksha Pal's questions See All

Is there ALDH positive Breast cancer stem cell lines commercially available ?

I need ALDH positive breast cancer stem cells for my reasearch work. Does any one can provide me the information that whether this cell line is available at ATCC or provided by other companies. I...

05 June 2016 8,271 4 View

Is telomerase activation a primary or secondary phenomenon in cancer?

07 August 2013 1,843 1 View

After keeping cells starved for 24 h cells are treated with activator or inhibitor, should the treatment be in serum free media or complete medium?

I want to check the effect of inhibitor on the gene expression after 24 hours. I grew the cells in serum free media for 24 hours then I give the treatment with the inhibitor and activator of the...

05 June 2013 5,522 8 View

How to avoid rapid pH fluctuation in DMEM medium?

Before storing the DMEM (sigma high glucose) at 4°C. I set the pH 7.2, and next day I checked the pH of the medium. After warming up at 37°C the pH rose to 7.8. What could be the reason and will...

03 April 2013 8,216 23 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to prediction 2D to 3D aptamer?

Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...

30 July 2024 6,794 0 View

Paul Jennings

There are protocols to isolate RNA and protein at the same time. But usually they are different.

Tejaswi Naidu

Why don't you divide your cells in to two parts and perform ELISA with one part and Isolate protein from from the other part. Because excess movement of cells may hamper you RNA Isolation.

Deeksha Pal

Thank you so much for your kind reply..