Can we incorporate unnatural amino acids into protein using software for graphical image purpose?

More Manikandan Santhanam's questions See All

How do we plot gain versus frequency plot in CST tool while designing an antenna?

I think there is no direct option to plot gain versus frequency in CST. kindly give solution

09 October 2023 8,738 3 View

The pixels of an image is scanned from left to right and from the top to bottom. each new pixel is predicted by the avg of the pixel above?

the pixels of an image is scanned from left to right and from the top to bottom. each new pixel is predicted by the avg of the pixel above and one to the left.

01 October 2023 4,525 1 View

Can anybody suggest how to make a hard mask from Ledit file for photo lithography?

I have only ledit file how to make hard mask for mask aligner. is this possible to do using laser writer or engraver. how to make pattern?

09 September 2023 903 1 View

Is there any non-denature approaches to solubilize protein aggregates or Triton insoluble protein aggregates?

I have isolated the Triton insoluble protein aggregates from the cell culture media and blood serum. I got the pellet (insoluble protein) in the final stage of the isolation process and it’s not...

21 March 2023 9,946 10 View

How to prepare input data for discrete wavelet transform?

i would like to do trend analysis for long time series data using multi resolution wavelet analysis. i am struck with preparing input data to analyze using discrete wavelet transform. i have tried...

21 December 2022 1,006 3 View

IPTG protein induction not working, How to resove this?

Hello all, I would like to express a gene of full-length human Tau (2N4R) into BL21 DE3+ strain of E. coli. I have sequenced the plasmid(pET15b) and it has the right sequence. The plasmid...

03 December 2022 754 2 View

Protocols to separate or isolate protein aggregates from cell culture media and Bio fluids?

I am working in the neurovegetative diseases. I want to isolate the extracellular protein aggregates from the cell culture media, serum, and Plasma. I have done it with cells and tissues before,...

05 September 2022 1,403 0 View

Is there any method to purify recombinant Tau protein without FPLC?

Hello Everyone, I am looking to purify Recombinant tau monomers for my studies. But we don't have FPLC system with us. I am wondering any methods available to purify without the FPLC system....

25 August 2022 3,386 7 View

Can anyone please suggest the best software for Petrov-Galerkin method?

I am working in numerical simulation and analysis of heat source models using Petrov-Galerkin method. I would expect the best and most suitable software for PG method please

24 August 2022 7,102 3 View

I didn't add PHA in my karyotyping Techniques, that's why no metaphase was found my pellet. Pls let me know if any solution is available or not ?

I have done a CA Techniques. I didnt have PHA so I was not added in culture setup. Therefore no metaphase was found my sample any possibility is available to get metaphase in sample pls let me know

09 August 2022 409 2 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Is it possible to use the Fused Deposition Modeling (FDM) to additively manufacture interconnected porous structure generation of >100-200 micrometer?

Usually, additive manufacturing techniques like SEBM, SLS, and SLM are used for interconnected porous lattice structure generation with sizes of >100–200 micrometers. Can the Fused Deposition...

09 August 2024 7,892 0 View

How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

How can I apply boundary conditions in an orthotropic steel deck numerical model using ABAQUS software?

I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...

08 August 2024 719 0 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Can you suggest reliable sources defining "3D mesh" and "3D city models"?

Dear fellow researchers, I am currently working on a paper where I need to provide a reliable reference that defines and distinguishes between 3D mesh models and 3D city models. Although I am...

06 August 2024 9,986 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Martin Klvana

If the non-natural amino acid is similar to any of the available ones, use the most similar one as a template and modify it.

Adriano Mollica

maestro by schrodinger has a large choice of unnatural aminoacids. you should be able to use just the editor if you don't have the full license.

Kiran Lokhande

You can use Academic Version of Maestro (Schrodinger) software to mutate/add existing amino acid with unnatural amino acid.