Cerebral infusion of 6-HDA does induce the lesions in rats. E.g., Neuroprotective effects of oral gallic acid against oxidative stress induced by 6-hydroxydopamine in rats. Food Chem. 2013 Jun 1;138(2-3):1028-33. doi: 10.1016/j.foodchem.2012.11.022. Epub 2012 Nov 16.
Yes you can prepare model for parkinson disease by intracerebral injection of 6- OHDA 2 to induce Parkinson in rats. Please go through this paper.
Redox Rep. 2005;10(2):103-9.
Involvement of nitric oxide in neurodegeneration: a study on the experimental models of Parkinson's disease.
Singh S1, Das T, Ravindran A, Chaturvedi RK, Shukla Y, Agarwal AK, Dikshit M.
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Abstract
The present study was undertaken to explore involvement of nitric oxide (NO) in the experimental models of Parkinson's disease. Neurodegeneration was induced by unilateral injections of 6-hydroxydopamine (6-OHDA) or lipopolysaccharide (LPS) in the right striatum. Lesions were functionally evaluated by amphetamine-induced asymmetrical behaviour and by decrease in the tyrosine hydroxylase (TH) immunostaining. An induction in the expression of iNOS and augmentation in nitrite content was observed in both the models. The extent of increase in iNOS expression was, however, different but the elevation in the nitrite content was comparable in both the models. The increase in iNOS expression inversely correlated with the tyrosine hydroxylase (TH) immunolabeling. Animals pretreated with a NOS inhibitor, N(G)-nitro-L-arginine methyl ester (L-NAME), exhibited complete protection against amphetamine induced rotations in both the models. Thus, augmented NO availability subsequent to iNOS induction seems to play an important role in the initial phase of neurodegeneration.
Dear Mr Pradip thanks 4 ur answer.
I'll be gr8ful to u if u provide me paper
What do you mean by developing? This is already the "classical model" of Parkinson's disease since 30 years
http://www.ncbi.nlm.nih.gov/pubmed/234524
By the way, keep well in mind positive and negative points of this 6-OHDA model
http://www.ncbi.nlm.nih.gov/pubmed/22108613
By developing I mean to create Parkinsonism in Rats using 6 OHDA
Heya Prafulla,
Yes you can develop parkinson like symptoms in rat by 6OHDA infusion. Take a look at the Romulo's science paper attached here. Also you can look at some other articles from Miguel Nicolelis lab at Duke Univ. where some graduate students and postdocs are using 6-OHDA for developing parkinsonism in rat. You can talk to Amol Yadava (graduate student) in the same lab who is currently working on developing parkinson disease in animal models.
hope this will help
good luck
http://www.sciencemag.org/content/323/5921/1578.full.pdf
Dear S. K Sharma,
can you please specify on what you based your statement that modeling PD is more difficult in rat than in mouse?
Respectfully,
N. Casadei
@S K Sharma Sir we don't have steriotaxic frame in King George's Medical University Lucknow thats why we are looking 4 alternate methods of inducing Parkinsonism
Thank you for your comment, I edited my post accordingly.
Hi Prafulla Chandra Tiwari,
For the 6-OHDA model the injections need to be precise, a steriotaxic frame would be of great importance. You could try finding a used a steriotaxic frame to buy.
We don;t have stereotaxic frame in KGMU and govt is not providing funds 4 it, thats why i've to go 4 intracerebral injection
What you want is an "intracisternal" (actually 4th ventricle) injection, using a 1/4" needle. Use 200ug of 6-OHDA.Br (calculated as the base), with 0.2 mg/ml ascorbic acid in 25 ul 0.85% saline: Hedreen JC, Chalmers JP Neuronal degeneration in rat brain induced by 6-hydroxydopamine; a histological and biochemical study. Brain Res. 47:1-36, 1972.
For additional remarks and cautions see also: Hedreen J Neuronal degeneration caused by lateral ventricular injection of 6-hydroxydopamine. I. The ventral premammillary nucleus and other cell groups. Brain Res. 157:129-135, 1978. (& also Brain Res Bull 5:425-436, 1980; further literature quoted in these two latter papers).
PMID:4345031 PMID:81096 PMID:6996790
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