Dear all,
I am wondering if my purified chaperone protein (Hsp104) is stable in -80 C. I tried to store it in for few days right after the AKTA-FPLC. I dry-freeze the AKTA products containing the target chaperones supplemented with the buffer containing tris, NaCl, mgCl2, 2.5% glycerol and BME. I am worried about the stability of chaperones and would be grateful if you provide some information or share your experience in this regard.
Thanks and regards,
Bikash
Instead of freeze-drying, why don't you just flash-freeze the purified protein in the liquid state in small aliquots and store it at -80 C? This avoids excessively high salt in the samples. If the protein is too dilute, use ultrafiltration to concentrate it before freezing.
Dear Dr. Adam,
Thank you for the information. I will proceed as suggested.
Hi
From my experience, increase glicerol concentration ~10%. The protein concentration will not directly affect your protein stability but protein concentration should be above 1.5 mg/ml
Jarek
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