No signal/bands observed on western blot for ERK signaling?

My question is regarding a western blot experiment that I am performing to observe pERK signaling by stimulation with VEGF ligand (VEGF165 and VEGF121) on MDAMB231 cells and SW620 cells (both...

30 January 2021 1,039 3 View

Cell line that does not express any of the VEGF receptors?

I am looking for VEGFR-negative cell lines, that either show very low or no expression at all of VEGF receptors for cell signalling assays.

01 November 2020 6,046 1 View

Is there a way to correlate one-dimensional netcdf data (like a time-series data) with the spatial gridded dataset (which is multi-dimensional)?

For eg. I have a netcdf file which is in the form of a time-series and the other data is a gridded .nc file for geopotential height. I want to correlate both of these datasets. Please guide me...

27 May 2020 8,133 3 View

I have doped ZnO nanoparticles using tungsten and antimony.1% doping..their atomic radius is different so doping would be possible or not?

i have analyzed samples using XRD, i got peaks with a slight peak shift for pure and doped material. no any addition peak was found. just XRD is enough to confirm the doping?

04 December 2019 2,030 3 View

I am working on a medical imaging research and if anyone knows where I could find CT scans of the brain with and without contrast agents?

I have looked up Cancer Imaging Archive but I need a bigger dataset. Can anyone help me out with some good places to look at for more images with and without contrast agents?

28 October 2019 4,219 3 View

Extracting smart health band data?

We need to download or extract health data such as heart rate, b.p, calories burned etc. from smart health-band. However, most of the bands are associated with their makers' app and do not provide...

03 June 2019 3,655 1 View

Can transwell assays be used to study protein transfer between two discrete populations of cells?

Hello, I was wondering if transwell assays with permeable supports could be used to measure protein transfer between two different populations of cells. If I used a small pore size (less than 3...

05 November 2018 8,364 1 View

Does the nature of ligand affect the crystallization process or rate of nucleation during the crystal growth ?

my ligand is completely soluble in DMSO but very less soluble in other organic solvents.. I tried many times to obtain the crystals of my ligand with mercury using cobinations of solvents like...

27 August 2018 7,971 15 View

I need help on deciding which journal to submit my publication?

Hello I am graduate student and need help deciding which journal to submit my article. The article pertains to surface and inter-facial properties of food oils. It is very engineering and physical...

05 March 2018 4,071 8 View

Where can I buy SH-SY5Y cells in Canada or US?

25 July 2016 763 4 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

How does GROMACS calculate the average structure of proteins?

I want to get a average structure from a pdb file containing multiple models. I used the command below: gmx cluster -f input.pdb -s topol.tpr -cutoff 20 -method gromos -av GROMACS...

25 February 2021 6,314 1 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View