Hello, I am working on protein detection sensor, and in one step, I need to incubate protein targets with the antibodies on the electrodes. However, antibodies are only functionalized on certain places of the electrodes with a width of 5 um, and length of 550 um, and it will be great if all proteins within the sample are within this area rather than the entire electrodes. So is there a way to do that? I am thinking of making a microchannel, but 5 um wide microchannel seems to be difficult to make (maybe cut it with FIB on PDMS?). Is there a easy way to make this microchannel? Many thanks!
Seems some critical info is missing. What are the electrodes made of? Is it glass? What about Silane Glass Treatment or polymerize a coating on it?
If the electrode is porous, we sometimes can use an air gap in the channel to place the sensor liquid. This can be done very precisely using a precision cap pump or syringe.
I also think that something is missing here. If you already immobilized a selective antibody on the electrode, a focussed incubation is not necessary at all. The antibodies will trap your antigens even from a larger volume. All other areas not coated with antibodies should be "blocked" by incubation with a blocking agent, such as 1% BSA (many other options). This avoids that your antigen binds to other areas.
Can be done. You can do it with any good laser engraver. See attached publications.
Article Continuous-flow microfluidic printing of proteins for array-...
Article A critical comparison of protein microarray fabrication technologies
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