Can we combine cDNA synthesis kits of different suppliers?

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Soner Öner-Sieben

Hey,

most likely it will work. If not, try purifying the first strand before moving on to the next step. By the way if you want to perform PCR you don''t need the second strand translated.

Best

Mei-Lan de Graaf

Hi Soner Öner-Sieben ,

Thank you for your answer. We're doing NGS so as far as I understand we do need the second strand. We are going to try to combine the 2 kits most likely