I am able to collect CSF from rat by one time puncture in cisterna magna but I have a need of any method or protocol by which I can collect CSF serially from one rat. Can anyone recommend a complete methdology?
Yes the method for multiple collection is published by transcutaneous cisternal puncture.....( you can try
Reference...Serial Cerebrospinal Fluid Collection in Rats for Pharmacokinetic
www.absorption.com/absorb.../serial-cerebrospinal-fluid.htmlShare9 May 2012 – Typically, CSF is collected terminally; i.e., one animal per time point. Serial collection of multiple CSF samples from the same animal offers the ...