Can we check gene disruption in yeast genome just by its colony pcr?

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No title

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Amy Klocko

You can do a quick check by colony PCR. If you want to publish your findings you'll need to do a more thorough test (sequencing).

Robert Adolf Brinzer

Depends on the size of your deletion.

Hamid Kian Gaikani

you can, but make sure you do have positive control for your PCR since colony PCR is tricky, and the false negative rate could be very high.