I have disrupted yeast gene in genome now I want to verify it without delaying it for overnight culture and then genome isolation so can I directly check it (gene disruption )from transformed yeast colony plate by colony pcr?
Amy Klocko
You can do a quick check by colony PCR. If you want to publish your findings you'll need to do a more thorough test (sequencing).
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Hamid Kian Gaikani
you can, but make sure you do have positive control for your PCR since colony PCR is tricky, and the false negative rate could be very high.
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