Can we apply CRISPR genome editing on cell line?

More Fadwa Aljoud's questions See All

Does anyone have a standard protocol for applying CRISPR in K562  cell line?(b- thalassemia)?

Greetings, I'm planing to do genome engineering by CRISPR in K562 cell line. so I'm currently writing my first grant proposal application ever. Does anyone have a standard protocol for applying...

07 August 2019 420 5 View

Contamination or something els?

I'm in shock right now! I checked my flasks this morning and found strange things init! This is my first time finding like this in my culture!! Although I follow a precise aspect technique in my...

06 July 2019 7,986 1 View

I need a list of the ISI for journals plz?

Does anyone knows how can get the recent list of the ISI for journals? and how can I search for the knowledge in?

01 February 2018 205 0 View

Urgent question : How I can keep Bax active ?

I want to keep Bax ( pro apoptotic member) continuously active in mouse model. Can any one suggests me a method of that? Thanks

04 May 2016 7,135 2 View

I have problem to get a high density by using SW840 cell line?

I have tried many times to setup cultures using this cells line (SW840) in 10 ml DMEM + 5 ml FBS + 500μl P/S and then incubate the culture in Co2 incubator at 37 c and 5 % of co2, but the...

02 March 2016 1,020 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

Does anyone have a standard protocol for applying CRISPR in K562 cell line?(b- thalassemia)?