Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Is there any tutorial for comsol general projections?

Hi, im trying to make a projection in a 3d comsol model. I have a non uniforme chanel and my intention is to get the average tempreture in the y-z cross section and se the average tempreture...

02 March 2021 4,624 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

I need dataset for recommandation system?

Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?

27 February 2021 7,063 7 View

How to estimate 2SLS with different instruments for different endogenous variables?

To be specific, I want to conduct an IV regression in stata with the following three equations. EQ1: Y1 ~Y2 Y3 X EQ2: Y2 ~ Z1 EQ3: Y3 ~ Z2 Both Y2 and Y3 are endogenous. However, Z1 and Z2 are...

23 February 2021 6,266 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

How to find out the problem of VMD software when i run it?

Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...

22 February 2021 8,211 3 View

First time secondary research, some points I should keep in mind?

I am conducting secondary research for my dissertation for undergraduation (Psychology) , what should I make sure my proposal consists of? Are there any tips I need to keep in mind?

22 February 2021 6,035 1 View

Sample modeling in ABAQUS software and its relationship with the real world?

About crack modeling in ABAQUS: Mode I (pure-tensile cracking) and Mode II (pure-shear cracking), these assumptions may be accruing in theoretical approach (ABAQUS) not practical in Lab....

19 February 2021 6,156 1 View

Making my research papers available for readers?

17 February 2021 8,533 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Can I heat my ELISA at 37C?

Hello, I am conducting a sandwich-type ELISA. It is to see if small molecules can interfere with a protein-protein interaction. As this interaction would occur in the human body, may I heat...

31 January 2021 4,271 3 View

The > 91 Years long collective IQ Blunder ?

Never before was there a bigger blemish. Namely: Not to collectively underastand Zwicky's discovery of Cryodynamics, as his sister to deterministic Thermodynamics came to be called later. Dec....

30 December 2020 8,300 5 View

Is GPD 2021 an official and real event?

I was invited to this conference organized by the Hazel pharmaceutical group in Prague 2021. I want to know if the event is real and if any other collage in the pharmacology/ drug discovery area...

25 December 2020 4,407 3 View

Will the spread of the new SARS-Cov-2 strain affect the efficacy of mRNA-based vaccines?

Recent studies have highlighted the isolation of a new strain of SARS-Cov-2. Could the new discovery compromise the effectiveness of the vaccination campaign ???

21 December 2020 7,516 4 View

Is there any database or website from where I can get a list of FDA approved drugs for treatment of certain urinary tract infection?

I want to know all prescription drugs which are FDA approved compounds for infection like Urinary Tract Infections along with their causative agents. Can you guide me about any resource, database...

17 December 2020 4,852 3 View

How will DeepMind AlphaFold2 solution of the core problem of protein structure prediction from sequence impact biology and biophysics?

AF2 may most strongly impact experimental determination of protein structure by multiple methods with possible benefits and negative impacts over time. Some areas may need to quickly pivot or...

13 December 2020 6,026 5 View

Molecular Dynamics simulation using BIOVIA Discovery Studio ?

Dear All, I want to do the MD simulation studies of protein-ligand complex using BIOVIA Discovery Studio. Can anyone suggest me any tutorial (Video) for the same and also for the result analysis....

09 December 2020 7,132 2 View

How to convert Material Studio forcefield and structure files into LAMMPS input files?

I have constructed some polymer structures using Material Studio. But I need LAMMPS input files for the simulation constructed from the Material Studio. Does anyone know how to get lammps input...

08 December 2020 251 1 View

Do you think that COVID-19 is completely disappear after vaccine discovery?

one year age and COVID-19 was the main subject that distracts the human minds. before few days, a vaccine was completing its trial experiment to be used in prevention among human beings. do you...

07 December 2020 9,422 8 View