I have been struggling with some plants in their in vitro propagation.
Can there be unusual methods in plant tissue culture? The other than the standard methods... Share me some citations please. Calling out to all Plant Tissue culture experts .
Dear @Gaurav Mudgal
Tissue culture protocols may vary depending on plant species. You have not mentioned it. Why are you interested in "unusual" method? Are you working on variant method? If so, you may standardize it. If you are interested in references, use correct key words, and google; you will find so many articles and references.
TY Arvind Sir, I am doing the G scholar way too but selective community would be here I guess... So I threw this question.. Thanks for the advice Sir.
Dear Gaurav Mudgal could you explain what do you consider unusual, or standard methods? Both are generalistic considerations. Axillary bud culture is probably the most used technique used in tissue culture; however, for those that follow somatic embryogenesis the use of axillary budding is rare. There're several morphogenic pathways (direct or indirect regeneration, somatic embryogenesis, adventitious buds,....), as well as culture methods (organs, tissues, cells,...) and regeneration systems (semisolid and liquid cultures, stationary or temporary,...). The election of a culture system mainly depends of your goals, plant species, and the available resources. Hope it help you.
By unusual I mean other than conventional explant type, plant growth regulators, supplements, technically, changes in conditioning physical factors .. and biotic factors. Likewise..
Dear Gaurav Mudgal your query has a difficult response. I suggest you that provide more details; otherwise I don't know what to say
OK, There are certain set standards known in culture conditionings, wrt temp, light, media, supplements. Are there many studies on blue sky researches ---- a well off beat than standards... let say just try adding X material (which has never been tried ever) this to your strategy) ..than what is one or other norm.
I suggest that you search by yourself, because you know what exactly you're looking for. If there's something "completely" new it's difficult to track. Good luck!!!!
There are just so many factors that you can change (light time, light intensity, tissue source, plant hormones types, hormone amounts, time on hormones, activated charcoal and other additives, basal medium amount, sugars or not, how much sugar, size of plant tissue, age of plant tissue, type of regeneration). It will really help to at least know the species and goals of the work.
Any variant of "standard" can be considered "unusual"
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