Also try to study the changes in dehydrogenase activity ,  structural and functional changes in soil microbial communities to link changes in soil organic matter with these parameters , in addition to nutrient pool.  

Binay , there could be  possibility  in  seasonal changes in soil organic matter , especially in forest soils , where soil microbial activity attains its peak during monsoon  period , coinciding with rains.

Dear Sir,

So do you suggest me to do estimate soil organic matter seasonally? It will be highly appreciated if you suggest some papers on soil organic matter for further reading.

Best,

Binay 

Sure , it will be in fitness of your study to do it , since there will be seasonal change in microbial activity . I will try to send to you some papers.

Also try to study the changes in dehydrogenase activity ,  structural and functional changes in soil microbial communities to link changes in soil organic matter with these parameters , in addition to nutrient pool.  

Thank you sir for providing insights. I am studying soil and leaf litter nutrients too but was unaware of dehydrogenase activity. Could you please suggest me the best method for measuring dehydrogenase activity?

i am doing Soil Organic Carbon now. i am taking SOC in single observation for my objectives. But to know the changes need to analysis for three seasons ie pre-monsoon, monsoon and post monsoon will be useful 

Dear Binay:

Dependent on the soil horizon of interest, it may be very difficult to detect changes in soil organic matter in your system, particularly seasonally. In colder climates, SOM can build to such a level that small changes are undetectable unless a prohibitive amount of samples are processed. One possibility is to look at the natural variability across the landscape and perform a sensitivity analysis to see how many replicate samples you would need to detect significant change.

-B

Dr.Bryan Dail,good suggestions.If the study site is a forest of central Himalayas,if the forest is well established and thick and the season is winter ,there is less scope for changes in organic matter.If the forest ground is exposed to sunlight and if  the soil temperature fluctuates in different seasons,then there is scope for seasonal changes in organic matter.