I WANT TO KNOW IF THE SEQUENCE OF PRIMERS USED FOR ISSR CAN ALSO BE USED FOR SSR FOR A PARTICULAR PLANT SPECIE LIKE VERNONIA AMYGDALINA
Okwuchi,
Primers for ISSR are rather universal because they have repeating sequence abundant in the studied genome, like (GTG)n or (ATT)n, when SSR primers are species (genus)-specific. Primers for ISSR are arbitrary (amplify random fragments between anonymous SSR), when SSR are locus-specific.
Okwuchi,
Primers for ISSR are rather universal because they have repeating sequence abundant in the studied genome, like (GTG)n or (ATT)n, when SSR primers are species (genus)-specific. Primers for ISSR are arbitrary (amplify random fragments between anonymous SSR), when SSR are locus-specific.
Both primers are different- ISSR primers r universal whereas SSR primers are plant genome specific
- Badges
- Science method