Can the RNA be run on DNA HS ChiP Bioanalyzer just after first strand synthesis? Or is it required to do the second strand synthesis as well (complete cDNA) before running it on the bioanalyzer?
I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...
07 August 2024 2,598 1 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Genome-wide association study (GWAS) Continuous traits: eg. Height Discrete traits: eg. Eye color
28 July 2024 286 0 View
Though much literature suggests fitting a Voigt profile on a LIBS peak, is it also possible to fit a Gaussian profile on the LIBS peak? I have 204 spectra files, and I am building a pipeline for...
23 July 2024 6,127 1 View
Please inform me about the International Conference on Plant Molecular Biology, or Plant Biology, scheduled for December 2024, January/February, or March 2025.
14 July 2024 3,120 7 View
need to know protocol for photoplate creation, styles, etc
11 July 2024 8,321 2 View
How can anyone develop a theoretical framework that successfully unifies general relativity and quantum mechanics? how this new theory should accurately describe gravitational interactions at...
10 July 2024 5,040 8 View
I am able to submit the code documents. Although my graph is 1, I would like to be in the 6E-05 graph range. Please, someone correct the error.
08 July 2024 5,770 0 View
I have tried to optimize the serum free dmem which is the best but i am afraid the secretory profile has too much variations.
03 July 2024 4,044 3 View
Please help me with one or more of the following, if possible: How does a client mentally process the trauma? What are the cognitive domains of processing? What measures are available to assess...
02 July 2024 6,263 0 View
When I examined the GIXRD data obtained recently, I observed that the sample treated with O2 plasma on the Cu surface deposited on Si chips showed increased intensity compared to the sample...
19 June 2024 6,900 5 View
Hi everyone, I am a beginner in ABAQUS and have modeled a turning operation using dynamic explicit. The results are kinda meaningful but it does not display the chip formation at all. 1-The...
14 June 2024 2,100 2 View
Hello fellow researchers, Why don't we see two rRNA peaks as is standard? Our sample was a total RNA prep from A549 cell line. We ran our samples on a pico chip for 2100 Agilent bioanalyzer...
28 May 2024 7,666 0 View
I want to work on microfluidic chip. Dispersed phase of alginate solution and continuous phase of Span 80 solution in combination with mineral oil or liquid paraffin. But I had a problem with the...
19 May 2024 1,790 7 View
I've been doing qPCRs a lot recently. I just wonder if we could link the qPCR probes we use onto surfaces so that we could creates some sort of chips. If not, is it also possible to link just the...
12 May 2024 5,397 0 View
I am trying to optimize ChIP for my cell lines. I am doing with WT cells. I keep checking sonication efficiency on an agarose gel. So if I take a new sample of the same cell line, do I have to do...
06 May 2024 7,321 0 View
I need to take blood from Tegu Lizard and to extract the RBC following nuclei isolation, sonication and ChIP assay: appreciate any help: 1) which blood-collection tubes are recommended? 2) how do...
03 April 2024 9,657 0 View
Hello Members, I am facing an issue with chip formation in a 3D turning process simulation, and I would greatly appreciate any insights or suggestions on how to address this problem. I have...
02 April 2024 2,110 0 View
I've been trying to extract RNA from mouse lung tissues (normal and tumour) and slowly improving the yield and purity measured via Nanodrop and Qubit (I'm consistently getting 260/280 ratios of ~2...
21 March 2024 113 5 View
Some times when sequencing on an ion torrent machine, there is a barcode that takes up a lot of data space, causing the sample data to be insufficient for analysis. But still on this Chip...
22 February 2024 2,743 0 View