What are the application of Fixed points thoery in Chemistry.?

It is said that fixed point theory has lot of applications not in the field of mathematics only but also in various areas. So I am looking for applications in Chemistry only..

26 February 2021 3,533 1 View

What are the important tips for writing review articles?

Important tips for writing a good review articles

24 February 2021 8,544 6 View

Level 1 pre-post multilevel regression: Panel data or ANOVA approach?

Dear colleagues, I am currently teaching myself in multilevel analysis using educational data. I found few to zero literature on basic analysis methods. In my context we frequently use level-1...

17 February 2021 9,378 5 View

How to extract RNA from brown adipose tissue(BAT)?

30 January 2021 6,610 3 View

How to take XRD of colloidal Ag NPs?

I have synthesized silver nanoparticles using sodium citrate as reducing agent. I have carried out XRD characterization by drop coating colloidal solution on the glass substrate ( since yield in...

27 January 2021 1,416 3 View

What is the ACTUAL COST OF A PUBLICATION??

Hi. It's 2021 January. Recently, I got to know that Elsevier has converted around 160 journals into some "Transformative Journals" and follow the recommendations under "Plan S"...

19 January 2021 9,985 2 View

Does anybody have the recipe for making a home made R electroporation buffer for Neon transfection systems?

I tried the recipes below in two cells lines: 1.DPBS 2.DPBS+MgCl2(1mM) 3.DPBS+Sucrose(250mM) 4.DPBS+MgCl2(1mM)+Sucrose(250mM) 5.R Buffer (Neon) the results showed that R buffer is the best one in...

06 January 2021 6,776 1 View

Does Lucifer Yellow interfere with miniEPSC/IPSC recordings?

I want to mark the neurons I am recording from during whole cell Patch clamp. I am thinking of using Lucifer Yellow. Does it increase noise during recording? What are the better alternatives to...

04 January 2021 7,791 3 View

How Learning in Multiagent Control System Using Graph Theory is useful ?

I would like to know application of learning and control in MAS setting. And where could I find simulation codes of learning in MAS control

04 January 2021 1,866 4 View

How to standardize and set parameters in the Puller sutter P-97 to get accepted pipette shape and resistance for whole cell recordings on DRG?

We are working on a P-97 puller and we are using it to do whole cell recording in DRG. We have 2 types of pipettes (borosilicate glass ID: 1.1 OD: 1.5 and ID: 0.86 OD: 1.5 ) as it showed in the...

04 January 2021 2,112 6 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

Droplet PCR in plastic chips always result an unexpected band, any suggestion of possible contaminations and how to improve?

I am doing droplet based digital PCR inside plastic chips. I can get the correct band, but I also consistently get an unexpected band, slightly shorter. I attached a typical gel image here....

18 February 2021 6,618 2 View

How do I check the quality of my viral RNA prior to metagenomic sequencing?

I am doing metagenomic sequencing without enrichment for an RNA virus. After my RNA is extracted from the blood I am doing library prep with the NEBNext Ultra directional RNA library prep kit for...

16 February 2021 5,101 2 View

RNA concentration: Nanodrop vs Agilent 2100 Bioanalyzer?

Hi guys. I make RNA by in vitro transcription and do cell transfection afterwards. I analyze RNA using Nanodrop and Bioanalyzer. But I found the concentration of RNA measured by these two machines...

05 February 2021 292 3 View

How to remove colour from DNA pellet during DNA extraction?

I am working with Amaranth accessions which are green to red in color and contain high phenolic compounds. Using the CTAB method with the slight modification I am trying to get high-quality DNA so...

18 January 2021 3,578 1 View

A three electrode sensor inkjet printed gets damaged in few hours of usage in a dynamic media flow chip, any solution for that?

We are using some biosensors which are inkjet printed and are used in the microfluidic chip where media is flowing at the flow rate of around 60 uL/min. However, after the continuous use of few...

05 January 2021 610 2 View

How to clean LEDs (InGaN on SiC) and Ti/Au electrode chips?

I need perfect cleanliness for further processes but I am not sure which cleaning method would be sufficient yet letting the layers intact.

01 December 2020 1,099 2 View

The algal cell always gather together when the cells concentration is high and those cells would block the microfluidic chips. How can i handle it?

I want to pump a high concentration algal cells into microfluidic chip, however those cells are always gather together forming a clusters to block the connection between Teflon tube and...

26 October 2020 5,366 2 View

Can any one tell me that how we represent RFID chip (Z=23-j224) with a discrete port in CST?

Dear All, I am facing trouble in making antenna for RFID tag. Just tell me that how a chip resistance represents by discrite port in CST? for example I am using NXP chip with...

22 October 2020 7,968 2 View

Have spin-based devices found any appilcation in medical science?

Spin-based devices such as magnonic chips, spin-torque oscillators mostly find their application in signal processing, computing, etc. Is there any research work focusing on developing magnonic...

17 October 2020 3,459 1 View