21 Questions 15 Answers 0 Followers
Questions related from Shilpa Patil
Hi, I'm curious about the exact organelle where ferroptosis takes place. Initially, I thought it was limited to the mitochondria and ER, but after reading a few papers, it seems it might occur in...
22 November 2023 2,242 2 View
I want to look at the viability of my cells from my primary cell isolation. I am planning to do a Hoechst - PI staining of these cells (which are in suspension) and look under the fluorescence...
19 August 2022 1,093 0 View
I was looking for expression of ribosomal subunit L10a in pancreas and it feels like there is difference in staining. I was wondering if the translation machinery in acinar vs ductal cells different?
12 July 2022 665 0 View
Can the RNA be run on DNA HS ChiP Bioanalyzer just after first strand synthesis? Or is it required to do the second strand synthesis as well (complete cDNA) before running it on the bioanalyzer?
14 June 2022 6,475 0 View
During RNA/nuclei isolations we usually have DTT or Beta mercaptoethanol in our buffers which basically reduces the disulfide bonds of the RNases thereby inhibiting them. My question is - since...
25 May 2022 5,115 1 View
The Bioanalyzer basically looks at the quality of rRNAs and generates the RIN value.. Is there a way to access the quality of mRNA or even pre-RNA?
24 May 2022 8,907 2 View
Both Beta-mercaptoethanol and DTT are needed by RNases for stability, thereby inhibiting RNase activity and preserving mRNA. But which one is better and do they both act in the same way?
24 May 2022 5,666 1 View
Hi, I want to perform a proteomics study on the organoids which are grown embedded in the Matrigel. I am looking for a defined protocol to isolate proteins from these organoids which helps get...
20 May 2022 5,396 2 View
We are trying to test the effect of insulin on cancer organoids. I am planning to try concentrations between 0 nM to 200 nM but I am open for suggestions. We are looking for acute responses,...
02 May 2022 7,543 3 View
I am planning to switch my media from Ad DMEM-F12 to Human Plasma Like Medium (HPLM) to grow our human pancreatic tumor organoids. Few components are different in these 2 medium and I would like...
09 March 2022 616 1 View
I am doing an experiment where I have to treat the organoids with insulin, harvest RNA and then perform RNA-seq. But I am not sure how long should the insulin treatment be to see an effect on...
14 February 2022 2,530 2 View
Is the a way to check the quality of nuclear RNA on agarose gel? Since there will not be any rRNA there, I was not sure if it can be run on a gel or the only way to assess the quality if by...
06 December 2021 274 9 View
I am performing snRNA Seq from mouse pancreas. Usually RNases is a major problem when working with pancreas. I was planning to use DEPC treated water to make solutions since DEPC treated water is...
19 November 2021 9,003 6 View
I want to look at the influence of certain compounds on the organoids. I am looking for a high-throughput assays using plate reader. One assay I could think of is to analyze proliferation by...
15 September 2021 338 7 View
We are planning to order L-WRN cell line to obtain wnt3a/R-spondin/Noggin conditioned media. We were thinking if we can buy these individual components separately if that is economical. I was...
09 September 2021 9,829 2 View
I need to look for the expression of certain proteins in organoids which are embedded in Matrigel. Is it possible to fix organoids in collagen or Matrigel and later stain the organoids for markers?
09 July 2021 4,244 3 View
When I give a list of genes regulated by my protein of interest as input in GSEA MSigDB, does the output of C3 TFT mean that my set of genes regulate these Transcription factors?
23 September 2018 8,822 5 View
I have the ChIP seq of H3K27me3 in my sample. I in analyzing in Galaxy. When I compare my bigwig files and bed files (after MACS peak calling), I see that there are many peaks which are appearing...
02 August 2018 7,449 0 View
Can anyone suggest a good H3K27Me3 antibody for western? I have tried cell signalling and Abcam. Abcam is relatively better. But can anyone suggest a more specific one?
30 November 2016 7,213 3 View
I need a very good quality RNA for RNA-Seq. When I run the RNA on 1% agarose gel the sample looks good. But the same sample on Bioanalyzer shows a low RIN value. Which method is more reliable to...
30 November 2016 5,524 3 View
I am new to the organoid work and any suggestions would be helpful. I want to treat my organoid with different compounds and look for many readouts (proliferation, invasion, viability, EMT,...
01 January 1970 2,280 2 View
