can the medium and all its components for culturing E14 mESCs be filtered without any undesirable effect?
STANDARD ES CELL MEDIA (600 ml) (F. Mariani 03/12/2003):
ES CELL PROTOCOLS (for ES Cells that are feeder independent)
473.4ml KNOCK-DMEM
90ml 15% FBS (HyClone Tested Lot)
6ml Pen-Strep (100X)
6ml Na Glutamine (100X)
12 ml LIF (50X)
6ml Na Pyruvate, 0.11mg/ml (100X)
6 ml Non-essential Amino Acids (100X)
0.6ml 2-mercaptoethanol, 5.5uM (1000X)
Hey,
in case you take a low potein binnding filtration unit it should not influence your medium especially because you have a lot of serum protein in the medium. So I guess it is no probleem. If you want to completely safe add LIF after filtration
regards Sven
Dear Alex,
Filtering media should be fairly safe, even after LIF addition. I guess it is possible that some filters may bind (and effectively filter out) some amount of LIF or serum proteins but I have never had a problem when occasionaly filtering ES cell media over 15 years.
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