11 November 2015 1 5K Report

I only used only one reference gene in PCR.

Can the Genorm or Bestkeeper be used to evaluate only one reference gene stability?

More Shen he's questions See All
Can the sample of the highest ΔCt value instead of highest CT value be chosen to serve as calibrators in qPCR analysis?

Commonly, the sample of the highest, lowest,and the mean CT value can be used as calibrators. ΔCt=Ct gene of interest - Ct internal control Can the sample of the highest ΔCt value instead of...

10 November 2015 8,798 4 View

Is it correct to calculate a ROC curve for data obtained with the -ddCt methods using a calibrator for the analysis of real-time PCR data?

We used ROC curve analysis to assess the discriminatory power of a microRNA in distinguishing patients from healthy controls. and I want to consul whether is it correct to calculate a ROC curve...

10 November 2015 3,357 3 View

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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What is the prepared reference material that can be used in inductively coupled plasma optical emission spectrometry (ICP-OES) to measure REE and U ?

What is the prepared reference material that can be used in the ICPE-9820 Shimadzu Japan instrument, which employs inductively coupled plasma optical emission spectrometry (ICP-OES) to measure...

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Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

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How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

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How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

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Which will be the best software for the Hydration shell analysis with molecular dynamics?

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Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

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