Shen he

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Questions related from Shen he

Can the sample of the highest ΔCt value instead of highest CT value be chosen to serve as calibrators in qPCR analysis?

Commonly, the sample of the highest, lowest,and the mean CT value can be used as calibrators. ΔCt=Ct gene of interest - Ct internal control Can the sample of the highest ΔCt value instead of...

11 November 2015 8,773 4 View

Is it correct to calculate a ROC curve for data obtained with the -ddCt methods using a calibrator for the analysis of real-time PCR data?

We used ROC curve analysis to assess the discriminatory power of a microRNA in distinguishing patients from healthy controls. and I want to consul whether is it correct to calculate a ROC curve...

11 November 2015 3,332 3 View

Can the Genorm or Bestkeeper be used to evaluate only one reference gene stability?

I only used only one reference gene in PCR. Can the Genorm or Bestkeeper be used to evaluate only one reference gene stability?

11 November 2015 4,580 1 View