Greetings,
In order to determine the degree of hydrolysis of protein isolates by the pH-stat method, we take the pK of the alpha-amino group of our proteins as 8 (according to the literature). However, our experimental conditions for the enzymatic hydrolysis will be at 37 ◦C for one sample and 70 ◦C for another one. Will this difference alter the significance of our study if we use the same pK in the formula for both of the samples?
Best,
Dr. Nabil Adrar
Anuraj Nayarisseri
pK is negative log to base 10 of dissociation. and dissociation is dependent on temperature. The pK mentioned in the (biochem)literature are values at 25 degrees.
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