I would like to know if there are any natural bioactive compound or other post-harvest treatments to prevent chilling injury, control decay, preserve fruit color and firmness and prolong the shelf life of strawberry fruit during cold storage
You can apply the Reducing Atmosphere Packaging technique which depends on the use of molecular hydrogen gas beside both carbon dioxide and nitrogen. The role of hydrogen is inhibiting the deterioration of pigments and antioxidants during storage period as well as an inhibition effect against the molds and yeasts.
You can find our published paper on the preservation of fresh cheese using this technique. Regarding the preservation of freshness attributes and extension of the shelf life of strawberry using this technique, we have actually a manuscript which is under review.
Washing berries is not an option because even sterilized water may contaminate them, and accelerate its deterioration. BUT a combination between applying ozone (O3) gas for a short time, and then preserving berries in a CO2/N2 atmosphere, may be a good idea, did you try that?
You can buy a small O3 equipment in aliexpress or alibaba (1-2 g O3 h-1), it cost only 30-40$, and you can try it:
1) inoculating fungi spores to strawberries and applying gas in intervals of time, during x time, and try with different cold temperatures.
2) or simply test a number of strawberries in individual hermetic boxes, in RT or cold chamber, injecting O3 in boxes, in short intervals of time for several days.
You can check with this, which is the protocol to extend the life span of strawberries, simply changing parameters of time, number of intervals, and temperature.