Edu assay by spectrophotometry means?

Dear all, Have any one performed edu assay by spectrophotometric means ? I'm looking for a kit for that purpose. I have Chinese kit for this purpose, I followed their protocol but the result is...

24 November 2020 1,766 3 View

How can i measure traces of Hg2+ spectrophotometrecally, ?

I want to measure a low concentration of Hg2+ in a solution spectrophotometrically, ia there an indicator or something i can use as an indicator for Hg2+ to color it and make it easy to measure

14 September 2020 3,575 3 View

How to do MTT for non-confluent cells?

Hi everyone, I'm doing MTT assay to test the cytotoxicity of 4 candidates on the BT-474 breast cancer cell line which rarely becomes confluent. started with 3K/well, 5K/well, 6.5K/well left the...

12 September 2020 3,034 8 View

Hello, I'm looking for colleagues researchers who use water modeling tools in their study in Africa?

As groundwater modeling, solute transport, surface water modeling or any other modeling used in water resource management

27 April 2020 8,390 18 View

Need a good master thesis topic in improving wireless network using AI techniques?

I need help plz, anybody can help me to find a good master thesis topic in the field of computer science/IT. The topic related to improving wireless network performance/security using one of...

02 January 2020 1,671 3 View

Is there a meta-analysis of Customer-Dominant Logic (CDL) empirical studies?

CDL is a recent perspective that advances service-dominant logic (SDL) even further towards considering the customer, and the customer context, as a foundation or centerpiece for business. In...

10 September 2018 5,392 2 View

How can I extract the land use from google earth?

Are there any software that make the land use extraction from google earth

22 February 2016 626 11 View

What are the fatigue symptoms?

01 January 1970 5,259 1 View

Body Mass Index Calculator

Body mass index (BMI) is a value derived from the mass (weight) and height of a person. The BMI is defined as the body mass divided by the square of the body height, and is universally expressed...

01 January 1970 8,305 16 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View