Dear researchers,
Lately in our lab (Unimore, Italy) we wanted to tag a Pseudomonas strain (Biocontrol) with gfp or mCherry in order to use it for localization assay, please could you help us, from where can we get the plasmid of gfp or mCherry with the cheapest way, for free if possible :)
Best regards
Fares
Omar Hammam Salloum
Addgene: Addgene is a valuable resource for obtaining plasmids used in research. They offer a wide variety of plasmids, including those expressing fluorescent proteins. You can find plasmids containing GFP and mCherry sequences on the Addgene website. Some plasmids are available for free, while others may have a nominal cost. Here are a few specific plasmids you might consider: EGFP-mCherry (Plasmid #193553):Expresses mEGFP-mCherry in mammalian cells. Available through Addgene for $851. Link to EGFP-mCherry plasmid mCherry (Plasmid #176016):Available from Rob Parton’s lab. Published in Journal of Cell Biology. Link to mCherry plasmid pmCherry-N1 GBP (GBP-mCherry) (Plasmid #162879):Contains the insert GFP Binding Protein. Published in Proceedings of the National Academy of Sciences. Link to pmCherry-N1 GBP plasmid Explore Addgene’s website for more options and availability. Collaborate and Share:Reach out to colleagues in your field or collaborate with other labs. Researchers often share plasmids within the scientific community. Literature and Protocols:Search for published protocols or articles where researchers have successfully used GFP and mCherry. Learn from their methods and adapt them to your specific needs.
Tagging your Pseudomonas strain with GFP or mCherry is a great approach for localization studies. Here are some options to obtain these plasmids:
Remember to cite the original authors when using plasmids obtained from repositories like Addgene. Good luck with your localization assays! 🌟🔬
0 votes
0 thanks
Fares Bellameche
Omar Hammam Salloum and Tom Masi Thank you so much for your time and for the very helpful advises !
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Filing
More Fares Bellameche's questions See All
Similar questions and discussions