Are there any published reports of immunofluorescence being used to to differentiate mouse strains in an allogeneic transplant system? For example, antibodies against H2b to detect C57BL/6 tissue from a Balb/c (H2d) host? MHC class I antibodies would probably be best, but any other strain-specific molecules that can be used to differentiate strains might work as well. I have found lots of antibodies claiming to detect H2d or H2b, but I'm hesitant to try them, as they need to be very specific for these haplotypes.
I think Thy1.1/1.2 can not be used in this case, since both mouse strains used have the same.
Thanks Matthew. I'm specifically interested in blood and lymphatic endothelium, so Thy1.1/1.2 and CD45.1/CD45.2 won't work for me, unfortunately.
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