Can someone share a standardized protocol for calpain detection through zymography?

05 May 2014 1 7K Report

I use casein zymography of stomach tissue but could't get the expression of calpain.

Don't know if you already got an answer or the right protocol, as your questions is not the latest one, but have you checked some typical publications working with calpains and zymography (see the links)?

http://www.ncbi.nlm.nih.gov/pubmed/7771786

http://www.ncbi.nlm.nih.gov/pubmed/10818754

http://www.ncbi.nlm.nih.gov/pubmed/18559257

Badges
Science topic

More Anil Kumar Chauhan's questions See All

Is there any biotin positive pancreatic cancer cell Line ?

Most of the cancer cells are thought to have biotin receptor. However, I am unable to find any pancreatic cancer cell line having this receptor except a single paper stating PANK-1 as a biotin...

08 September 2017 606 1 View

Is it possible that some natural compound have selective toxicity towords drug resistant cells but not parent cells ?

Hello Everyone, I am using a natural compound against MCF-7/Parend and MCF-7/ADR cell lines, interestingly, I found my compound is showing toxicity against MCF-7/ADR (doxorubicin-resistant) cells...

10 November 2016 835 6 View

Which LPS is more toxic E. coli or Salmonella ?

Hello Everyone, I have a query in my mind regarding the toxicity of two LPS types i.e. E. coli and Salmonella. I'll highly appreciate some expert comments on this issue. Thank You

02 March 2016 7,283 1 View

How to dissolve nisin from sigma?

I have sigma nisin 2.5% (balance sodium chloride and denatured milk solids) according to specification it dissolves in water, but I'm getting some precipitation while dissolving in water or 5%...

03 April 2015 9,833 7 View

Is it possible to dilute the serum for cytokine and biochemical examinations ?

I have to examine various cytokines as well as liver enzymes from serum, but I have a very small amount, so i want to know whether it can be used after diluting it? if yes then what should be the...

02 March 2015 3,105 7 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What should Berlin do as a city to become as impactful as London and Paris in World Football?

Please go through my Abstract. I can also share a proposed Thesis Outline.

04 August 2024 2,077 0 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View