Is there any biotin positive pancreatic cancer cell Line ?

Most of the cancer cells are thought to have biotin receptor. However, I am unable to find any pancreatic cancer cell line having this receptor except a single paper stating PANK-1 as a biotin...

08 September 2017 523 1 View

Is it possible that some natural compound have selective toxicity towords drug resistant cells but not parent cells ?

10 November 2016 760 6 View

How to dissolve nisin from sigma?

I have sigma nisin 2.5% (balance sodium chloride and denatured milk solids) according to specification it dissolves in water, but I'm getting some precipitation while dissolving in water or 5%...

03 April 2015 9,725 7 View

Is it possible to dilute the serum for cytokine and biochemical examinations ?

I have to examine various cytokines as well as liver enzymes from serum, but I have a very small amount, so i want to know whether it can be used after diluting it? if yes then what should be the...

02 March 2015 3,031 7 View

Can someone share a standardized protocol for calpain detection through zymography?

I use casein zymography of stomach tissue but could't get the expression of calpain.

04 May 2014 6,994 1 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

Can anyone help me with the administration of the "Mind the Gap" Scale for satisfaction with transition to adult care?

We are working on a study focused on adolescents' transition to adult care and are looking for a validated measure for satisfaction with the transition. So far the only measure that we have came...

28 February 2021 2,338 1 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

First time secondary research, some points I should keep in mind?

I am conducting secondary research for my dissertation for undergraduation (Psychology) , what should I make sure my proposal consists of? Are there any tips I need to keep in mind?

22 February 2021 6,035 1 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

Which vial septa the right one to be used for HPLC?

This is the unanswered question stored very long in my mind and till now I could not find a right answer. Google search educated me that the red surface is the PTFE material compatible to wider...

21 February 2021 546 3 View

How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

19 February 2021 7,447 2 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

What/How to focus on with regard to research publication?

I'm an academician for past 10 years and love to continue with the same, a question pops-up at in mind from time to time, what to focus more on? Conference papers, Journal paper, book chapters,...

09 February 2021 1,009 5 View