Please suggest me best software program for designing allele specific primers.
Hi,
U could go through this article and use the link generated by them.
http://www.biomedcentral.com/1471-2164/8/275
Plus, Batch primer three is good too.
http://probes.pw.usda.gov/batchprimer3/overview.html
I hope it helps.
Primer3 - online tool
http://biotools.umassmed.edu/bioapps/primer3_www.cgi
PerlPrimer
http://perlprimer.sourceforge.net/
Generunner - Analyze your oligos
http://www.generunner.net/
WebSat - online tool http://wsmartins.net/websat/
PrimerBLAST
http://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?LINK_LOC=BlastHome
SP-DESIGNER http://www2.sophia.inra.fr/urih/sophia_mart/sp_designer/info_sp_designer.php
You could try WASP:
http://bioinfo.biotec.or.th/WASP
Primer Express
T-ARMS technique
http://rjptonline.org/AbstractView.aspx?PID=2018-11-8-74
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