I am targeting to evolve my E. coli strain to improve its organic acid tolerance. Currently, my strain has MIC of up to 0.1 % only. Higher than that, my strain won't grow at all. It would be preferable if the method is fast.
Thank you Dr. Mejia for the interesting reference. However, I am looking for a method that would not take 60,000 generations of the strain in order to get my desired mutant. It would be preferable to have a faster method, say in less than 60 days I will be able to get the desired mutant.