How can I calculate parameters of pseudo-second model for adsorption processes?

07 December 2020 3,541 9 View

How to implement the GLCM (gray levels co-occurrence matrix) matrix to determine parameters such as entropy, kurtosis, and others,using python+OpenCv?

How to implement the GLCM (gray levels co-occurrence matrix) matrix to determine parameters such as entropy,kurtosis, among others of an image, using OpenCV and scikit-image libraries?

26 July 2018 5,722 1 View

Has anybody built a survey questionnaire with already validated constructs from other surveys?

HI, do you have experience with using already validated questions (for various constructs, e.g. construct to assess risk behavior, construct to assess school performance) in your own survey...

05 November 2017 6,342 3 View

Can I use mixed methods if i have only quantitative data?

28 September 2017 6,553 6 View

Do you know any module to run a test to compare two matrices (same size) from different populations? I know some tests, but...

I am not an expert in multivariate analysis, son I need your help. I have many covariance matrices related to the items of a score. I have information from many populations. I estimated the...

09 August 2017 9,074 2 View

Do you know any module to run a test to compare two matrices (same size) from different populations? I know some tests, but...

I am not an expert in multivariate analysis, son I need your help. I have many covariance matrices related to the items of a score. I have information from many populations. I estimated the...

07 August 2017 5,126 2 View

What is the theoretical maximum for the energy efficiency in cathodoluminescence?

Reportedly, zinc sufide (ZnS) doped with Ag or Al or Cu is the most energy-efficient scintillator: one estimate says it's 30 %, that is, for an optimized composition (doping, crystal size, etc.) a...

28 December 2015 2,829 3 View

Can anyone assist me with the sample size calculation for school based survey in a stratified two-stage cluster sample design?

It is a cross-sectional study to find out associations between specific factors and smoking behavior. Target population: 13 to 15 years old adolescents in school settings; the population size...

11 December 2015 5,229 2 View

Do you have any knowledge with (Poisson-Logit Hurdle Regression) in Stata?

09 July 2014 3,637 2 View

Do you have any knowledge with (Poisson-Logit Hurdle Regression) in Stata?

06 July 2014 6,468 2 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

What are the possible effects of COVID -19 Vaccine/s on the persons already recovered from the disease?

Persons recovered from COVID -19 are having antibodies against the virus inside their bodies. They are having T cells already sensitized to the antigens of the virus. If they are vaccinated (may...

12 February 2021 4,106 22 View

What is the reason, we got both test and control band on LFA in negative sample?

I am working on Lateral flow assay for virus detection by carbon conjugated polyclonal antibody. We got a high intense band in both test and control line on LFA in negative sample used as buffer....

11 February 2021 6,558 2 View