I am interested in finding a PCR-RFLP protocol for haemophilia A that can be used in the screening of the most frequent mutations in the FVIII gene.
About PCR-RFLP there is an article:
https://www.ncbi.nlm.nih.gov/pubmed/19150994
but in our lab my colleague performs IS-PCR for inv22 and inv1
Rossetti etal, 2008
and you have long range PCR:
Liu et al, 1998
Thank you for the quick response!
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