Long Distance PCR (LD-PCR) - problems implementing a protocol?

Hello, Does anyone have experience with LD-PCR implementation and optimization? I have been trying to implement a LD-PCR protocol in our Molecular Biology lab, but it seems it does not amplify...

10 November 2018 3,930 6 View

Reference sequence for the coagulation factor 8 gene

Can someone please provide the reference sequence for the coagulation factor 8 gene? It is located on the long arm of the X chromosome (X-q28). I am trying to allign some primers to detect...

04 May 2017 7,043 3 View

Can someone provide a PCR-RFLP protocol for haemophilia A?

I am interested in finding a PCR-RFLP protocol for haemophilia A that can be used in the screening of the most frequent mutations in the FVIII gene.

10 November 2016 1,222 2 View

Is anyone interested in becoming a Peer-Reviewer for a student journal?

Hello, I am the Scientific Editor of The International Journal of Medical Students (www.ijms.info). We publish original articles, reviews, short communications and case reports written by medical...

01 January 1970 3,197 8 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

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02 March 2021 3,060 3 View

Is it possible to do piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch?

How to do Piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch? Piezoelectric coupling between the rectangular substrate and rectangular...

02 March 2021 9,833 3 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

Can I follow Researchers from Teaching and testing areas?

I want Researchers from Educational Measurement and Evaluation, relating to teaching, learning , academic performance and test validation

18 February 2021 4,757 13 View

Does qualitative research using closed and open ended survey and semi structured interviews qualify as triangulation?

I am using qualitative research to look at employee motivation. I will use a closed and open ended survey (for biodata information and general views to motivation) and semi structured interviews...

18 February 2021 436 11 View

Should I include less important items in con-joint survey experiment?

I plan to do a conjoint survey experiment in which I randomly change attributes of hypothetical job candidates and show two types of potential candidates with different attributes. Then, I ask...

16 February 2021 5,812 1 View