Can someone point to a reference characterizing the electrophysiological properties of the MOUSE dopaminergic neurons in the SNc?

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Why does inorganic phosphorus in labile pool cannot be transfered to stable pool directly?

In swat, epic, or any other models to calculate inorganic phosphorus cycle, why inorganic phosphorus in active pool can be transfered to stable pool, but inorganic phosphorus in labile pool can...

28 February 2024 1,530 4 View

Why is there a disagreement in partial charge calculation from Autodock and PDB2PQR?

I am docking a protein structure at different pHs. After obtaining the PDB structure, I protonate the protein at different pHs using pdb2pqr, download the structure, then use Autodock vina to dock...

07 February 2024 6,446 0 View

Can lentivectors be used for transient transfection in cell lines other than HEK?

Hi all, We have a lentiviral vector with a gene of interest that we would like to express. There is no requirement for stable cell line construction, so we will only be transfecting transiently....

29 November 2023 2,802 6 View

How do a splice correction of a spectrum with spliceCorrection in R language?

I have a spectrum CSV file and I have tried using the spliceCorrection function in R package "prospect" to fix it, but the result is not good. Can anyone please guide me on a method (Software) to...

24 September 2023 1,392 0 View

Why is 50% methanol used for protein A regeneration?

In most protocols, 50% methanol is used for protein A regeneration, followed by storage, usually in water/buffer/20% ethanol. In my experience, it is critical to apply a 50% methanol wash so the...

29 August 2023 2,511 3 View

Antibody expression - low amounts of heavy chain and full length monomers?

Hi all, I am trying to produce an antibody from a plasmid in Freestyle CHOS cells. The plasmid is a HC/furin/p2A/LC vector. The cells were transiently transfected during the exponential phase and...

12 July 2023 2,425 5 View

Time constant for mammalian cell electroporation?

For a project we needed to use a specific device (Bio-Rad Gene pulser) to electroporate mammalian cells. We set up the instrument with the following settings: Voltage = 1.9kV (we had to use this...

02 July 2023 7,753 3 View

Can FITC-labeled peptides be purified other than HPLC?

Hello all, I recently started to use peptides for cellular studies. Unfortunately, when I was designing the peptides I did not have a FITC tag to them (the peptide was synthesized by an external...

31 May 2023 1,689 3 View

No or low protein expression after transfection of a cloned gene- also low A260/230 plasmid ratio?

Hello, We have been trying to insert peptide tags (

07 March 2023 425 6 View

Error in Discovery studio, how to solve?

Hello all, I'm using Discovery studio 2019 to dock protein-protein, while "An error was encountered during execution of ViewResults.ds_pl.", then the dock failed. As it works quite well with the...

30 November 2022 829 4 View

An animal in not a rock (e.g., Koch 2013), it has an internal constitution that can be modified?

Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...

07 August 2024 648 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Project/Experimental assistance ？?

Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...

05 August 2024 5,829 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Are the GABAergic neurons electrophysiologically equal in all brain areas?

Firing in GABAergic neurons has been characterized in several areas of the brain, but patterns are the same?

04 August 2024 4,090 0 View

How to draw the five chelate rings of [Co(EDTA)]- on this picture?

Is this circle one of the rings?

03 August 2024 5,988 0 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Information is Stored by Context?

While living in Boston, which has a fine metro system, there were times that I would exit from underground at the wrong location, thereby expecting a particular visual scene only to be momentarily...

17 July 2024 3,237 10 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized?

What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized - I have few of them listed and can be updated in this...

13 July 2024 2,431 2 View