Miaomiao Mao

7 Questions 10 Answers 0 Followers

Questions related from Miaomiao Mao

How to measure membrane capacitance in cell-attached mode/outside-out patch etc.?

Hi, Does anyone know how to measure membrane capacitance (hence membrane area) in cell-attached mode or outside/inside-out patches for the area of the patch in the pipette? I came across papers...

17 August 2020 4,749 3 View

Can you compare AMPA/NMDA ratio between mouse strains?

I've read that you can only compare AMPA/NMDA ratio before and after drug treatment as the value itself doesn't mean anything. Is this true? Can you compare AMPA/NMDA ratio between mouse strains...

16 April 2018 3,712 1 View

Protocols for doing calcium imaging with acute brain slices without perfusion of oxygenated ACSF during imaging?

Hi, I'm new to calcium imaging but have been doing electrophysiology (whole-cell patch clamping for a few years). As our imaging system does not have a perfusion system and no carbogen gas...

01 July 2016 5,127 4 View

How do I test receptor-receptor coupling in acute brain slices functionally?

Is there a way to test receptor-receptor coupling in acute brain slices (mouse)? I suspect physical interactions between an ionotropic receptor and a metabotropic receptor in the postsynaptic...

13 March 2015 1,615 3 View

Can someone point to a reference characterizing the electrophysiological properties of the MOUSE dopaminergic neurons in the SNc?

All of the papers I've found so far are on rats. I need some reference for the electrophysiological properties of the dopaminergic neurons in the substantia nigra pars compacta in the MOUSE. Thanks!

26 February 2015 1,108 2 View

How does holding potential work in cell-attached mode?

I'm doing cell-attached recordings with external ACSF in the pipette and hoping to see some currents in voltage clamp mode. The aim is to see whether adding glycine/GABA to the pipette would...

04 October 2013 2,256 4 View

What is the best way to determine whether chloride is depolarizing/hyperpolarizing in a neuron without imaging and using perforated patch?

I want to either measure the intracellular chloride concentration in neurons in acute brain stem slices or determine whether chloride is depolarizing or hyperpolarizing with external application...

20 May 2013 3,918 19 View