Miaomiao Mao

5 Questions 10 Answers 0 Followers

Questions related from Miaomiao Mao

Can you compare AMPA/NMDA ratio between mouse strains?

I've read that you can only compare AMPA/NMDA ratio before and after drug treatment as the value itself doesn't mean anything. Is this true? Can you compare AMPA/NMDA ratio between mouse strains...

16 April 2018 3,657 1 View

Protocols for doing calcium imaging with acute brain slices without perfusion of oxygenated ACSF during imaging?

Hi, I'm new to calcium imaging but have been doing electrophysiology (whole-cell patch clamping for a few years). As our imaging system does not have a perfusion system and no carbogen gas...

01 July 2016 5,100 4 View

How do I test receptor-receptor coupling in acute brain slices functionally?

Is there a way to test receptor-receptor coupling in acute brain slices (mouse)? I suspect physical interactions between an ionotropic receptor and a metabotropic receptor in the postsynaptic...

13 March 2015 1,592 3 View

How does holding potential work in cell-attached mode?

I'm doing cell-attached recordings with external ACSF in the pipette and hoping to see some currents in voltage clamp mode. The aim is to see whether adding glycine/GABA to the pipette would...

04 October 2013 2,219 4 View

What is the best way to determine whether chloride is depolarizing/hyperpolarizing in a neuron without imaging and using perforated patch?

I want to either measure the intracellular chloride concentration in neurons in acute brain stem slices or determine whether chloride is depolarizing or hyperpolarizing with external application...

20 May 2013 3,879 19 View